Key findings
- PRP increases migration of adipose-derived stem cells (ADSCs) in vitro, by up to 3x
- Higher PRP concentrations seemed to correlate with higher migration
- PRP appeared to upregulate key proteins linked to cell movement (FAK, paxillin, vinculin)
- PRP alters ADSC cytoskeletal structure and spreading
PRP Likely Boosts Stem Cell Movement for Muscle Repair
Skeletal muscle injuries are a common setback for athletes, often interrupting rigorous training regimens and competition schedules. In recent years, both platelet-rich plasma (PRP) and adipose-derived stem cell (ADSC) therapies have attracted a lot of attention. As you likely know, PRP is rich in growth factors and signaling molecules, while ADSCs have shown the ability to self-renew and become various orthopedic cell types.
In this study, published in JSRMs Regenerative Therapy, researchers set out to investigate whether PRP could enhance the movement (migration) of ADSCs, an important step for tissue repair and regeneration. ADSCs were isolated from the subcutaneous fat of Sprague Dawley rats, then exposed to PRP in the lab to observe the behavior and signaling changes.
The Findings and Key Regenerative Pathways
To assess the effects, the researchers used a series of in vitro assays. In wound healing, migration, and cell spreading tests, ADSCs treated with PRP demonstrated up to 300% better movement and spreading compared to untreated cells.
Further analysis revealed that PRP treatment increased the levels of both total and phosphorylated forms of focal adhesion kinase (FAK) and paxillin, as well as vinculin, proteins that regulate how cells adhere and move, which is critical for the repair process.
Immunofluorescence staining was used to examine the cytoskeleton of the ADSCs, focusing on filamentous actin (F-actin) structures essential for cell shape and movement. PRP treatment led to notable changes in the distribution of F-actin, indicating cytoskeletal rearrangement in these cells.
To confirm the importance of FAK and paxillin, the researchers used siRNA to selectively decrease their levels in the cells. After siRNA knockdown, migration dropped to 120% of control vs. 300% in unmodified PRP-treated cells, suggesting direct dependence on FAK/paxilin for migration.
Implications for Regenerative Therapies
Altogether, the findings suggest that PRP can enhance the migration and spreading of adipose-derived stem cells, supporting the idea that combining PRP and ADSCs could be beneficial in regenerative medicine, particularly for muscle repair.
It’s exciting, but it’s also important to note that this was done in vitro with rat cells and requires further in vivo studies to measure the clinical translation to humans.
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